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KMID : 0380020030180030165
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 3 p.165 ~ p.169
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DPPH Radical Scavenging Effect and in vitro Lipid Peroxidation Inhibition by Portulaca oleracea
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Lee Hee-Jung
Lee Bum-Jong
Lee Dong-Seok
Seo Young-Wan
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Abstract
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The antioxidative activity of Portulaca oleracea was tested using in vitro experimental models. Antioxidative activities were determined by measuring DPPH radical scavenging activity and lipid peroxide using 2-thiobarbituric and (TBA). The crude extract was sequentially partitioned with n-hexane, 15% aq. MeOH, EtOAc, n-BuOH, . A remarkable antioxidative effect was observed in the EtOAc and n-BuOH fractions. The DPPH radical scavenging effect (=17.90 /ml) of the n-BuOH soluble fraction was comparable with that of the natural antioxidant, -tocopherol (=6.99 /ml) and the inhibition effect of lipid peroxidation in mouse liver homogenate was similar to that of the natural antioxidant, L-ascorbic acid at a concentration of 1.0 mg/ml to 5 mg/ml
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KEYWORD
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Portulaca oleracea, inhibitory effect of lipid peroxidation, DPPH radical scavenging activity
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